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. Author manuscript; available in PMC: 2017 Sep 4.
Published in final edited form as: Dev Biol. 2017 Jun 16;429(1):321–334. doi: 10.1016/j.ydbio.2017.06.013

Fig. 2.

Fig. 2

Early cranial neural crest (CNC) cell specification and migration are not affected in miR-27 morphants. (A) Lateral view of Tg(sox10(7.2):mRFP)vu234 embryos injected either with MO-ctl or MO27 showing cranial neural crest cell streams populating the pharyngeal arches at 18hpf and 24hpf. Anterior is to the left and posterior is to the right. Neural crest streams are numbered from 1 to 3. The indicated ratio represents the number of embryos with the represented phenotype/total number of observed embryos. (B, C) Expression of the neural crest cell marker, sox10, and CNC marker dlx2a in MO-ctl and MO27 embryos at 16 hpf and 22 hpf detected by whole-mount in situ hybridization. Dorsal view of embryos with anterior side to the top.