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Predicted secondary structures of the type 1b DNA fragment. Alternative secondary structures predicted for type 1b DNA by mfold using constraints (A) 33, 90, 161, 173, (B) 33, 118, 125 and (C) 55, 62, 90, 173. The positions of major cleavage sites are indicated by arrows. (D) Optimal structure of the type 1b DNA fragment predicted by mfold. The calculated free energies for each structure at 1 M NaCl and 37°C are shown.
