Figure 2. Nucleolar translocation of progerin upon MG132 treatment.

1. Subnuclear distribution of progerin (red) and PML (green) after MG132 treatment of HGPS fibroblasts. Cells cultured with 5 μM MG132 for 24 h show staining of progerin and PML in intranuclear foci.
2. MG132 induces the translocation of PML (green), emerin (green), p53 (red), 26S proteasome subunit (red), and ubiquitin (red) into nucleoli of HGPS and control fibroblasts cultured in the presence of 5 μM MG132 for 24 h (relative to DMSO‐treated cells: control). Nucleoli were labeled with fibrillarin antibodies and nuclei with DAPI (blue). The merged images are shown.

Data information: Data in (A,B) are representative of six independent experiments. The experiments were performed on fibroblasts of HGPS patients and healthy subjects matched for age and passage. Scale bars, 5 μm.