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. 2017 Jul 3;9(9):1294–1313. doi: 10.15252/emmm.201607315

Figure EV2. Lamin B1/B2, emerin, ubiquitin, and 26S proteasome but neither lamin A/C nor Nup153 is included within PML‐NBs in HGPS fibroblasts.

Figure EV2

  1. Colocalization of lamin B1/B2 and emerin with PML in HGPS fibroblast nuclei. Immunofluorescence images of primary dermal fibroblasts from an individual with HGPS (left panels) and a healthy individual (control) (right panels) stained with DAPI (blue), PML (green), and antibodies to lamin B1/B2, emerin, lamin A, lamin C, and Nup‐153 (red). The merged images are shown. There are no PML‐NBs labeling with lamin A (red), lamin C (red), or Nup‐153 (red) antibodies. Scale bar, 5 μm.
  2. Confocal images of lateral (XY) sections (upper panel; scale bar, 2 μm) and axial (XZ) sections along the A, B, and C axis (lower panels; scale bar, 400 nm) of an HGPS fibroblast nucleus stained with lamin B1/B2 (red) and PML (green) antibodies.
  3. Immunofluorescence images showing colocalization of ubiquitin (red) and 26S proteasome (red) with PML (green) in the nucleus of HGPS fibroblasts. Nucleus was labeled with DAPI (blue). The merged images are shown. Scale bar, 5 μm.
Data information: Experiments in (A–C) were performed on four different cell lines showing the same results.