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. 2017 Jul 3;9(9):1294–1313. doi: 10.15252/emmm.201607315

Figure 7. MG132 reduces progerin and SRSF‐1 levels in Lmna G609G/G609G mice muscle.

Figure 7

  1. Western blotting evaluation of lamin A/C, progerin, actin, GAPDH, and SRSF‐1 in gastrocnemius muscle lysate of Lmna G609G/G609G mice treated with 1 μg/kg (mice 1 and 2) or 10 μg/kg (mice 3 and 4) MG132. The results are shown as four successive pairs (left: DMSO‐treated (0 μg/kg) and right: MG132‐treated (1 or 10 μg/kg) muscles, each corresponding to a single mouse).
  2. Lamin A‐, progerin‐, lamin C‐, and SRSF‐1‐specific bands, corresponding to DMSO‐treated left muscle (−) and 1 μg/kg MG132‐treated right muscle (+), were quantified by ImageJ software and their expression levels were normalized to GAPDH values.
  3. Lamin A‐, progerin‐, lamin C‐, and SRSF‐1‐specific bands, corresponding to DMSO‐treated left muscle (−) and 10 μg/kg MG132‐treated right muscle (+), were quantified by ImageJ software, and their expression levels were normalized to GAPDH values.
Data information: Results are expressed as mean ± SEM, n = 5, Student's t‐test, *P < 0.05, **P < 0.01, MG132‐treated vs. DMSO‐treated mice muscle; the exact P‐values are indicated in Appendix Table S1.