Skip to main content
. 2017 Jul 27;9(9):1263–1278. doi: 10.15252/emmm.201607066

Figure 1. Decreased ADK expression and increased level of intracellular adenosine in endothelial cells upon hypoxia.

Figure 1

  1. Biochemistry of the transmethylation reaction.
  2. Real‐time PCR analysis of ADK mRNA levels in HUVECs exposed to hypoxia for the indicated time periods. Results are from four independent experiments.
  3. Western blot analysis of ADK expression in HUVECs exposed to hypoxia for the indicated time periods. Images are representative from three independent experiments.
  4. Real‐time PCR analysis of ENT1 and ENT2 mRNA levels in HUVECs exposed to hypoxia for the indicated time periods. Results are from four independent experiments.
  5. Quantification of intracellular adenosine by high‐performance liquid chromatography (HPLC) in HUVECs exposed to hypoxia for the indicated time periods. Results are from four independent experiments.
Data information: For all bar graphs, data are the mean ± SD, *P < 0.05 and **P < 0.01 for the indicated comparisons; one‐way ANOVA with Tukey's post‐hoc test. The exact P‐values are specified in Appendix Table S1. Source data are available online for this figure.