Figure 7. Increased expression of angiogenic genes by intracellular adenosine.

• A
  Real‐time PCR analysis of angiogenic gene expression in control and ADK KD HUVECs. Results are from four independent experiments.
• B
  Representative images of VEGFR2 immunohistochemical staining on aortic sections of ADK^WT and ADK^VEC‐KD mice (n = 3 mice per group). The arrows indicate VEGFR2‐positive areas.
• C
  Immunofluorescent staining analysis of CD31 and VEGFR2 on sections of GC muscle at day 7 after hindlimb ischemia (n = 7 mice per group).
• D–F
  Confluent serum‐starved cells were incubated with VEGF‐A at 50 ng/ml. Western blot analysis of VEGFR2, Akt, P70, ERK, and their phosphorylated forms in: (D) control and ADK KD HUVECs, (E) vehicle and 5‐iodotubercidin (ITU)‐treated HUVECs, or (F) MAECs. Images are representative from three independent experiments.
• G
  Endothelial migration in control and ADK KD HUVECs treated with or without SU1498 at 1 μM and cultured in a transwell plate for 16 h. Results are from four independent experiments.
• H
  Endothelial sprouting of control and ADK KD HUVECs treated with or without SU1498 at 1 μM in a spheroid assay (n = 10 spheroids per group).

Data information: For all bar graphs, data are the means ± SD, *P < 0.05, and **P < 0.01; unpaired, two‐tailed Student's t‐test for (A and C); one‐way ANOVA with Tukey's post‐hoc test for (G and H). The exact P‐values are specified in Appendix Table S1. Source data are available online for this figure.