Skip to main content
. 2017 Jul 27;9(9):1263–1278. doi: 10.15252/emmm.201607066

Figure 8. The involvement of DNA hypomethylation or adenosine receptor in VEGFR2 expression.

Figure 8

  • A
    Western blots of endothelial cell VEGFR2. HUVECs were first infected with adenoviral ADK shRNA or control shRNA and then treated 6 h later with 5‐aza‐dC at 2 μM for 48 h. Results are from three independent experiments.
  • B
    Western blots of VEGFR2 expression in HUVECs treated with CGS 21680 at 5 μM or NECA at 5 μM for 24 h. Results are from four independent experiments.
  • C
    Western blot analysis of VEGFR2, p‐Akt, and Akt in control and ADK KD HUVECs in the presence of ZM 241385 (5 μM), MRS 1754 (5 μM), or both ZM 241385 and MRS 1754 for 24 h. Results are from four independent experiments.
  • D
    Western blot analysis of VEGFR2, p‐Akt, and Akt in control and ADK KD HUVECs transduced with either siA2AR or siA2BR or both. Results are from four independent experiments.
  • E, F
    Schematic of the proposed mechanisms for transcriptional regulation of angiogenic factors before (E) and after (F) ADK KD or inhibition. SAM indicates S‐adenosylmethionine; DNMT, DNA methyltransferase; SAH, S‐adenosylhomocysteine; SAHH, SAH hydrolase; AF, angiogenic factor; C, cytosine; mC, methylated cytosine.
Data information: For all bar graphs, data are the mean ± SD, *P < 0.05, and **P < 0.01 for indicated comparisons; one‐way ANOVA with Tukey's post‐hoc test for (A and B); unpaired, two‐tailed Student's t‐test for (C and D). The exact P‐values are specified in Appendix Table S1. Source data are available online for this figure.