FIGURE 3.

Hirsutine does not inhibit subgenomic reporter replicon activity. (A) Schematic representation of the replicon system, which uses DGL2 and DGL2-mut. DGL2-mut was inserted in non-replicative mutation into RNA-dependent RNA polymerase (RdRp). CMVp, cytomegalovirus promoter; Gluc, secretory Gaussia luciferase; IRES, internal ribosome entry site; GAA, mutation in the active center for RdRp; and Rib, ribozyme sequence. (B) Replicon plasmids DGL2 and DGL2-mut were transfected to A549 cells in the presence of 10 μM hirsutine. After 24 and 72 h, luciferase activity in the culture supernatant was analyzed. Each data point represents the mean ± standard deviation from triplicate experiments.