Fig. 1.

Schematic representation of the method for generation of low-density neuronal cultures. a NSCs/NPCs are seeded at the density of 4 × 10⁵ cells/well on uncoated coverslips. After 90 s, cell suspension is withdrawn and NSCs/NPCs are cultured overnight in mTeSR™1 medium followed by culture in Neurobasal medium. b During the first 24 h, cells start proliferating, extending lamellipodia and migrating. c Cells are differentiated for at least 4 weeks in Neurobasal medium (see Materials and Methods).