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. 2017 Sep 4;3:17043. doi: 10.1038/cddiscovery.2017.43

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Copyright © 2017 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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NOLC1 interacts with TRF2 in 293T cells. (a) In vivo Flag-tag pull-down analysis. Whole cell extracts of 293T cells transfected with Flag-NOLC1 or Flag-tagged-mock were immunoprecipitated with anti-Flag beads followed by mass spectrometric peptide sequencing. Both NOLC1 and TRF2 were identified. (b) MS identified TRF2 peptides. (c) In vivo binding of NOLC1 and TRF2. Lysates of 293T cells transfected with Flag-NOLC1 and GFP-TRF2 were immunoprecipitated with an anti-Flag monoclonal antibody and subjected to western blotting with anti-Flag and anti-GFP antibodies. (d) Reciprocal examination of the physical interaction between NOLC1 and TRF2. Immunoprecipitates obtained using an anti-TRF2 or anti-NOLC1 antibody were subjected to western blotting using anti-NOLC1 and anti-TRF2 antibodies.