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. 2017 Jul 21;7(3):226–230. doi: 10.3892/br.2017.948

Figure 2.

Figure 2.

Essential necroptosis proteins detected by western blotting. (A and B) TZA increased the expression levels of p-RIP-3 and p-MLKL, and Nec-1 and DPI exposure reversed this effect. (C and D) Quantification of p-RIP-3 and p-MLKL are presented as mean ± standard deviation. *P<0.05 vs. TZA group; #P<0.05 vs. control group. TZA, tumor necrosis factor-α + z-VAD-fmk + antimycin A; p-RIP-3, phosphorylated receptor-interacting protein kinase 3; p-MLKL, phosphorylated mixed-lineage kinase domain-like; Nec-1, necrostatin-1; DPI, diphenyleneiodonium.