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ROS content detected by flow cytometry in different groups. TZA increased ROS production in the HK-2 cells, whereas Nec-1 and DPI each inhibited ROS production. ROS, reactive oxygen species; TZA, tumor necrosis factor-α + z-VAD-fmk + antimycin A; Nec-1, necrostatin-1; DPI, diphenyleneiodonium; DCFH-DA, dichlorodihydrofluorescein diacetate.
