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DARPin CAR-T cells are cytotoxic to Her2-expressing target cells in vitro. CAR-T cells were co-cultured for 24 h with MDA.MB.468, MDA.MB.231, SKOV3, or MDA.MB.468.Her2 target cells at 1:1, 3:1, 5:1, or 10:1 effector-to-target ratios. Cytotoxicity against each target cell type was measured in triplicate and shown in the figure (n = 3, mean ± SEM; NS, not significant; *p < 0.05; **p < 0.01; ***p < 0.001).
