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. 2001 Aug 1;29(15):3172–3180. doi: 10.1093/nar/29.15.3172

Figure 3.

Figure 3

BLM is cleaved by caspase-3 in vitro. Western blot analysis of control HeLa extract (lane 1), HeLa extract treated with TNF-α (10 ng/ml) and cycloheximide (20 µM) for 8 h (lane 2); HeLa nuclear extract (HNE) (50 µg total protein/lane) treated with no caspase-3 (lane 3) and with 30 ng caspase-3 in the absence (lane 4) or presence (lane 5) of the caspase inhibitor acetyl-Asp-Glu-Val-Asp-aldehyde; recombinant BLM (10 ng) mock treated (–) (lane 6) or treated (lane 7) with 30 ng caspase-3. Treatment with caspase-3 was for 30 min at 30°C. The western blot was probed with anti-C terminal BLM or anti-PARP antibodies, as indicated.