Figure 4.

Immunohistochemical Detection of Free Fatty Acid receptor 4 (FFA4) in Mouse Lung. Antigen-retrieved, formalin-fixed paraffin-embedded lung tissue slices obtained from wild-type mice were incubated with anti-FFA4 antibodies [48]. They were incubated subsequently with Alexa 488-conjugated secondary antibody (green) and cy3-conjugated anti α-actin (to detect smooth muscle) (red). Following further washes, samples were placed on coverslips with a mounting medium containing the nuclear stain DAPI (blue). Images were taken using a confocal microscope. FFA4 appears to be expressed predominantly within the epithelial layer.