Figure 4. Phenotypic approaches to discover antitrypanosomatid compounds.
Various lifecycle stages can be used for the purpose of hit discovery that range from insect forms to host-stage forms in animal models. The different technologies that can be used for phenotypic assays depend on the parasite form and stage and have specific advantages and disadvantages. Examples of compounds whose antitrypanosomal activity was detected using insect forms, in vitro host-stage forms and animal models are shown.