Table 1.
Summary of α7 nAChR (α7) Antibodies Used.
| Company (Alphabetical Order) | Catalog No. | Lot No. | Reported Species Reactivity | Reported Epitope Target on α7 nAChR Sequence | Western Blot/Immunofluorescence Data | Monoclonal or Polyclonal | Secondary Antibody Target | # Citations (CiteAb) | # Rat Citations (CiteAb) |
|---|---|---|---|---|---|---|---|---|---|
| Abcam | ab23832a | GR89867-1 | h, r, m | 1–100 α7 Amino acid sequence | −/− | Polyclonal | Rabbit | 17 | 4 |
| EMD Millipore | AB15332 | 2630253 | m | 300–400 α7 Amino acid sequence | +/+b | Polyclonal | Rabbit | 0 | 0 |
| EMD Millipore | MABN529 | Q2459834 | h, m | Cytoplasmic domain | −/− | Monoclonal | Mouse | 0 | 0 |
| Novus Biologicals | NBP1-79948 | QC0391-42133 | h, r, m | N-terminal domain | +/++ | Polyclonal | Rabbit | 0 | 0 |
| Novus Biologicals | NBP1-79948 | QC42753-42341 | h, r, m | N-terminal domain | −/NA | Polyclonal | Rabbit | 0 | 0 |
| Santa Cruz Biotech. | sc-5544a (H-302) | D2214 | h, r, m | 367–502 α7 Amino acid sequence | −/− | Polyclonal | Rabbit | 31 | 6 |
| Santa Cruz Biotech. | sc-58607 (MAb 319)a | L0312 | h, r, m, c | Cytoplasmic domain | −/− | Monoclonal | Rat | 5 Sigma, 3 Santa Cruz | 1 Santa Cruz |
| Sigma-Aldrich | M220 (MAb 306)a | 040K4705 | h, r, c | 380–400 α7 Amino acid sequence | +/+ | Monoclonal | MouseTotal citations | 1066 | 415 |
Reported species reactivity and epitope locations are taken from the manufacturer’s literature. Key to western blot/immunofluorescence data: +/+ indicates positive western blot data showing antibody recognition of both α7 and α7-GFP chimeras and a significant difference in immunofluorescence between transfected and untransfected cells but with significant background; +/++ indicates positive western blot data showing antibody recognition of both α7 and α7-GFP chimeras and a significant difference in immunofluorescence between transfected and untransfected cells but with low background; +/– indicates positive western blot result but negative immunofluorescence staining; –/– indicates negative immunodetection using both applications; –/NA indicates negative western blot result and unavailability of immunofluorescence data. Abbreviations: h, human; r, rat; m, mouse; c, chicken; nAChR, nicotinic acetylcholine receptor; GFP, green fluorescent protein.
Previously evaluated in the literature using mouse knockouts.
Detected a band appropriate for rat α7nAChR on blots, but did not detect rat α7nAChR-GFP.