Fig. 5.

Yap deficiency activated Bnip3-required mitophagy. A-D. The change of t-FUNDC1, p-FUNDC1, t-Parkin, p-Parkin and Bnip3. Yap loss upregulated Bnip3 expression but had no role in other mitophagy receptors. E. Bnip3 upregulation was associated with the mitophagy activation. The siRNA was used to knockdown the expression of Bnip3. In Yap-deleted HepG2 cells, more LC3II migrated on the surface of mitochondria. In the amplification panel, we observed more foci of mitochondria and lysosome. However, this change was blocked once knockdown of Bnip3. F-I. Bnip3-required mitophagy was associated with CaMKII/cofilin phosphorylation. Western blots indicated that both of p-CaMKII and p-cofilin were suppressed once silence of Bnip3. J. Inhibition of Bnip3-required mitophagy reversed the F-actin and lamellipodia in Yap-deleted HepG2 cells. The F-actin and lamellipodia images were obtained under black-and-white vision. In the amplification panel, the yellow arrows indicated the lamellipodia. *P < 0.05 vs. control group; #P < 0.05 vs. shRNA-Yap group. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.).