Figure 3.

DNRX is co-localized with Scribble in the nervous system. A–A″, staining for scribble::gfp knock-in fly embryo with anti-DNRX (blue) and GFP is the spontaneous green fluorescence, showing that DNRX and Scribble are co-localized in the central nervous system. B–B″, third instar larvae brains of scribble::gfp staining with anti-DNRX (red) and GFP is the spontaneous green fluorescence, showing that DNRX and Scribble are co-localized in the central nervous system especially concentrated at mushroom body. C–C‴, confocal images of the third instar larvae neuromuscular junction at muscles 6/7 staining with anti-DNRX (blue), anti-DLG (red), and GFP is the spontaneous green fluorescence in scribble::gfp integrated with the DNRX overexpression fly, showing that DNRX and Scribble were co-localized in neuromuscular junction. D–D″, scribble::gfp knock-in fly adult brain staining with anti-DNRX (red) and GFP are the spontaneous green fluorescence, showing that DNRX and Scribble are co-localized in the central nervous system. E–E‴, epithelial cell staining with anti-DNRX, anti-DLG (red), and GFP is the spontaneous green fluorescence, showing that DNRX and Scribble are not co-localized at epithelial cells. Scale bar, 50 (A–A″), 50 (B–B″), 20 (C–C‴), 100 (D–D″), and 20 μm (E–E‴).