Figure 4.

Taking advantage of symmetry to improve stability. ThreeFoil structure (PDB entry 3PG0) is shown from a side view, perpendicular to the 3-fold axis of symmetry (top) and rotated 90° to look along the axis of symmetry toward the capping β-hairpin triplets (bottom). The first, second, and third subdomains are shown in magenta, cyan, and yellow, respectively. Sites of mutations used for MMut1 (K6V/K53V/K100V, A15V/A62V/A109V, and D38P/D85P/D132P) and MMut2 (same as MMut1 plus D2N/D49N/D96N) are shown as thick sticks.