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. 2017 Jul 18;292(35):14473–14485. doi: 10.1074/jbc.M116.765578

Figure 6.

Figure 6.

MCU is required for oxidative stress-induced mitochondrial calcium uptake. A, knockdown of IP3R blocked the lethality induced by MCU overexpression in muscle. Genotypes are as follows: Mef-Gal4/+, UAS-MCU-FLAG/+;Mef-Gal4/+, Mef-Gal4/UAS-IP3R RNAi, and UAS-MCU-FLAG/+;Mef-Gal4/UAS-IP3R RNAi. B and C, averaged traces (B) and quantitative analysis (C) of [Ca2+]mito signals from the flies that express IP3R RNAi, Drosophila MCU, or Drosophila MCU and IP3R RNAi upon treatment with 35 mm t-BuOOH (n = 5–9). Genotypes are as follows: Mef-Gal4,UAS-MTRP/+ (Mef>), Mef-Gal4,UAS-MTRP/UAS-IP3R RNAi (Mef> IP3R RNAi), UAS-MCU-FLAG/+;Mef-Gal4,UAS-MTRP/+ (Mef>MCU), and UAS-MCU-FLAG/+;Mef-Gal4, UAS-MTRP/UAS-IP3R RNAi (Mef>MCU, IP3R RNAi). D and E, averaged traces (D) and quantitative analysis (E) of [Ca2+]mito signals from S2 sells transfected with luciferase dsRNA or IP3R dsRNA upon treatment with 3 mm H2O2 (n = 4). F, eye-specific expression of transgenes using Gmr-Gal4. In clockwise order from the top left panel, genotypes are as follows: Gmr-Gal4/+, Gmr-Gal4/UAS-Sod1, Gmr-Gal4/UAS-Sod1 RNAi, Gmr-Gal4/UAS-Sod1 RNAi;UAS-MCU-Myc/+, Gmr-Gal4/UAS-Sod1;UAS-MCU-Myc/+, and Gmr-Gal4/+;UAS-MCU-Myc/+. G, genetic interactions among MCU, IP3R, and Sod1. Genotypes are as follows from left to right: UAS-Sod1 RNAi/+;Mef-Gal4/+, UAS-MCU-FLAG/UAS-Sod1 RNAi;Mef-Gal4/+, UAS-MCU-FLAG/+;Mef-Gal4/UAS-IP3R RNAi, and UAS-MCU-FLAG/UAS-Sod1 RNAi;Mef-Gal4/UAS-IP3R RNAi. ***, p < 0.001. Error bars, S.D.