Figure 1.

Expression of CCR6 and HuR on T helper cells and Treg cells. Naive CD4⁺ T cells were isolated from spleen of WT mice and activated or polarized as described under “Experimental procedures.” Activation (Act), Th1, Th2, Th17, and Treg cells were analyzed after 5 days of cell culture. Expressions of CCR6 on T helper cells and Treg cells were analyzed by flow cytometry (A) and qRT-PCR (B). C, HuR protein levels in CD4⁺ T cells were assessed by Western blot analysis. D, summary of HuR protein level in Th17 cells compared with that of activated CD4⁺ T cells. One of three independent experiments is shown in A and C. Data in B and D are derived from at least three independent experiments and presented as mean ± S.E. (error bars). *, p < 0.05; **, p < 0.01.