Figure 4.

MiR-335 negatively regulates Ccr6 mRNA. A, TargetScan analysis prediction that miR-335, miR-197, and miR-409 have potential binding sites on the CCR6 3′-UTR. B, qRT-PCR analysis shows that knock-out of HuR increased the level of miR-335 and miR-409 in Th17 cells. C, overexpression of miR-335 reduced the activity of luciferase reporter containing the CCR6 3′-UTR. HeLa cells were transfected with a luciferase reporter construct containing CCR6 3′-UTR, in which overexpression of miR-335 decreased the luciferase activity. Data in B and C were derived from at least three independent experiments. D, sketch diagram of a luciferase reporter construct containing CCR6 3′-UTR. *, p < 0.05; **, p < 0.01. Error bars, S.E.