Figure 5.

Knock-out of HuR impairs Th17 cell migration in vitro and in vivo. A, Transwell chemotaxis assay of WT and HuR KO Th17 cells was performed in the presence of CCL20 (CCR6 ligand). The migrated cells were analyzed at 3 h of culture. HuR deficiency significantly reduced Th17 cell migration to CCL20. B, CCR6 mRNA level in splenic CD4⁺ T cells from WT and HuR KO and mice with EAE. C, number of CD4⁺CCR6⁺ cells in the CNS of WT and HuR KO mice with EAE measured by flow cytometric analysis. D, CCR6 expression on CNS monocytes from recipients of WT and HuR KO Th17 cells. The results shown in A–D are a summary of at least three independent experiments. All data are expressed as the mean ± S.E. (error bars). *, p < 0.05; **, p < 0.01.