Figure 6.

After Treg cell depletion, the severity of EAE is lower in HuR KO than in WT mice. A, EAE in WT and HuR KO mice without Treg cell depletion. Each group contains six mice. CCR6 levels on WT and HuR KO Treg cells were analyzed by qRT-PCR (B), Western blots (C), and flow cytometry (D). E, EAE in WT and HuR KO mice in which Treg cells were depleted by anti-CD25 10 days earlier. Each group contains 11 mice. One of two representative experiments is shown in A, C, D, and E. Data in B are derived from at least three independent experiments and presented as mean ± S.E. (error bars). *, p < 0.05; **, p < 0.01.