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. 2017 Jul 6;292(35):14544–14555. doi: 10.1074/jbc.M117.785014

Figure 1.

Figure 1.

IL-4 stimulation increased Cox-1 protein expression. A, real-time PCR of Ptgs-1 expression in murine BMDMs stimulated with or without IL-4 and 0.1% DMSO for 20 h. B, representative Western immunoblot of Cox-1 and Gapdh expression in murine BMDMs stimulated with or without IL-4 for 20 h. C, representative Western immunoblot of Cox-2 and Gapdh expression in murine BMDMs stimulated with or without IL-4 or LPS for 20 h (n = 1–2 biological replicates per group). D, 15d-PGJ2 production 20 h post IL-4 stimulation. E, percentage of CD206+Arg-1+F480+CD11b+ murine BMDMs stimulated with or without IL-4 relative to untreated control. F, Western immunoblots of COX-1 and GAPDH from human PBMC-derived macrophages stimulated with or without human IL-4. Unpaired two-tailed t test. **, p < 0.01; ****, p < 0.0001. n = 4–7 biological replicates per group. All experimental data are expressed as mean ± S.E.