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. 2017 Jul 6;292(35):14544–14555. doi: 10.1074/jbc.M117.785014

Figure 2.

Figure 2.

Cox-1 is post-transcriptionally up-regulated by IL-4. A, real-time PCR on Arg-1 expression in murine BMDMs stimulated with or without IL-4 and DMSO or actinomycin D. B, representative Western immunoblot for Cox-1 and Gapdh expression in murine BMDMs stimulated with or without IL-4 and DMSO or actinomycin D. C, protein density of Cox-1 relative to DMSO untreated control. D, representative western immunoblot for Cox-1 and Gapdh expression in murine BMDMs stimulated with or without IL-4 and DMSO or cycloheximide. E, bioinformatic analysis of TOP sequences in murine and human PTGS-1 mRNA. F, polysome profile on BMDMs stimulated with or without IL-4 and torin 1 for Ptgs-1 and Rpl-13a mRNA expression, n = 1 per group. Two-way ANOVA with Fisher's LSD test. *, p < 0.05; **, p < 0.01; ****, p < 0.0001. n = 2–4 biological replicates per group. All experimental data are expressed as mean ± S.E.