Figure 3.

Cox-1 expression is controlled by the Fes/Akt/mTORC signaling pathway. A, representative Western immunoblot for Cox-1 and Gapdh expression in murine BMDMs stimulated with or without IL-4 and DMSO or herbimycin A. B, representative Western immunoblot for Cox-1 and Gapdh expression in murine BMDMs stimulated with or without IL-4 and DMSO or Akt inhibitor VIII. C, representative Western immunoblot for Cox-1 and Gapdh expression in murine BMDMs stimulated with or without IL-4 and DMSO or torin 1. D, production of 15d-PGJ₂ from murine BMDMs stimulated with or without IL-4 (20 h) and DMSO or torin 1. E, percentage CD206⁺Arg-1⁺F480⁺CD11b⁺ murine BMDMs stimulated with or without IL-4 or torin 1 relative to DMSO untreated control. F, Western immunoblots of COX-1 and GAPDH from human PBMC-derived macrophages stimulated with or without human IL-4 and DMSO or torin 1. G, representative Western immunoblot for Cox-1 and Gapdh expression in Cas9 murine BMDMs transduced with either Ntc or Tsc2-specific CRISPR gRNA and stimulated with or without IL-4 (n = 1 per group). Two-way ANOVA with Fisher's LSD test. *, p < 0.05; **, p < 0.01; ****, p < 0.0001. n = 3–6 biological replicates per group. All experimental data are expressed as mean ± S.E.