Figure 5.

AMPK activation in vivo delayed parasitic clearance. A, representative Western immunoblot for Cox-1 and Gapdh expression in the jejunum of mice treated with either water + PBS or metformin + PBS day 8 post-inoculation with infective larvae. B, number of adult worms per mouse based on treatment day 8 post-inoculation. C, correlation between Cox-1 protein expression and number of adult worms per mouse based on treatment day eight post-inoculation. D, real-time PCR on Arg-1 expression in the jejunum of infected mice based on treatment day 8 post-inoculation. ROUT method (Q = 5.0%). One-way ANOVA with Fisher's LSD test. Linear regression. *, p < 0.05; **, p < 0.01. n = 5–6 biological replicates per group. All experimental data are expressed as mean ± S.E.