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. 2017 Jul 5;292(35):14576–14586. doi: 10.1074/jbc.M117.796011

Figure 2.

Figure 2.

Neither nej1Δ nor nej1-V338A rescues the resection defect in rad50Δ mutant cells. Resection of DNA 0.15 and 4.8 kb away from the HO DSB was measured by percentage of ssDNA 0 and 6 h after DSB induction in wild type (JC3585), rad50Δ (JC3882), nej1Δ rad50Δ (JC3887), nej1-V338A rad50Δ (JC3897), and yku70Δ rad50Δ (JC3878) (A and B) and in nej1Δ (JC3884), nej1-V338A (JC3896), and lif1Δ rad50Δ (JC3907) cells (C–F) in G1 stage of the cell cycle. Error bars represent standard error of three replicates. Significance was determined using one-tailed, unpaired Student's t test where mutants were compared with wild-type cells for changes in resection levels (**, p < 0.01). G, ChIP assay as described in Fig. 1, E and F, was performed to measure the enrichment of Rad50-3HA at a DSB in wild type (JC3306), nej1Δ (JC3307), and nej1-V338A cells (JC3347). Error bars represent the standard deviation of three replicates. Significance was determined using one-tailed, unpaired Student's t test. All mutants were compared with wild-type cells (*, p < 0.05).