Figure 1.

Time course of PS/PC oxidation. A and B, oxidation of 500 μm 18:1, 18:2, or 18:3 PS/PC vesicles as indicated with either 200 μm CuSO₄, 2 mm H₂O₂ (panel A) or 10 μm CuSO₄ (panel B) in pH 7.4 Tris buffer at 37 °C monitored by conjugated diene formation at 234 nm. The effect of including 100 μm BHT on the oxidation of 18:3 vesicles is shown by open squares in panel A. C, light scattering at 320 nm produced by increasing concentrations of 18:3 OxPS/PC (●) or 18:3 PS/PC (○) in Tris buffer, pH 7.4. Lines drawn through the data are least squares fits.