Figure 5.

Requirements for OxPS/PC inactivation of ZPI and ZPI-PZ complex. A and B, progress curves of the inactivation of 150 nm free ZPI in calcium-free buffer (panel A) or 150 nm ZPI-PZ complex in buffer plus 5 mm Ca²⁺ (panel B) by 50 μm phospholipid vesicles of the following compositions: 18:3 OxPS/PC (●); 18:3 OxPC (■); 18:3 OxPS/PC (▿) in the presence of 10 mm KI; 18:3 OxPS/PC in the presence of 100 μm BHT (▵) and 18:1 OxPS/PC (♢). Vesicles (500 μm) were oxidized with Cu²⁺/H₂O₂ and diluted 10-fold into ZPI. Lines are empirically drawn through the data points.