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. 2017 Jun 27;292(35):14695–14703. doi: 10.1074/jbc.M117.798298

Figure 3.

Figure 3.

RNA-dependent interaction between METTL6 and SARS. HEK293T cells were transfected with either FLAG-METTL6 (M6) or FLAG-METTL6-ΔSAM (M6Δ). Equal amounts (protein level) of cell extracts were immunoprecipitated (IP) with either nonspecific IgG as a control or FLAG antibody, and treated with either buffer (−), RNase (+), or DNase (+), followed by SDS-PAGE, blotting, and probing with SARS or FLAG antibodies. Blots with Input cell extracts were probed with SARS and β-actin antibodies.

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