Figure 3.
RNA-dependent interaction between METTL6 and SARS. HEK293T cells were transfected with either FLAG-METTL6 (M6) or FLAG-METTL6-ΔSAM (M6Δ). Equal amounts (protein level) of cell extracts were immunoprecipitated (IP) with either nonspecific IgG as a control or FLAG antibody, and treated with either buffer (−), RNase (+), or DNase (+), followed by SDS-PAGE, blotting, and probing with SARS or FLAG antibodies. Blots with Input cell extracts were probed with SARS and β-actin antibodies.