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Effect of mutating the putative N-glycosylation sites of hRFVT-3 on transport function. HuTu-80 cells transiently expressing GFP (vector alone), wild type (WT), or N-glycosylation mutants were used for RF uptake determinations. [3H]RF (14 nM) uptake was performed in K-R buffer (pH 7.4) at 37°C for 3 min. Data are means ± SE of multiple determinations from at least four independent uptake experiments. *P < 0.01, **P < 0.05.
