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Effect of mutating the putative N-glycosylation sites of hRFVT-3 on cell surface expression of hRFVT-3 in HuTu-80 cells. Coexpression of GFP-hRFVT-3 (WT) or N-glycosylation mutants (left) along with DsRed-ER (middle), displayed overlaid images (right). The transiently coexpressing HuTu-80 cells were imaged 48 h posttransfection.
