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Effect of mutating the predicted substrate interaction residues in the of hRFVT-3 protein on RF uptake. Uptake of [3H]RF (14 nM) by HuTu-80 cells transiently expressing GFP (vector alone), GFP-hRFVT-3 (WT), and the substrate interaction residue mutants was performed in K-R buffer (pH 7.4) at 37°C for 3 min. Data are means ± SE of multiple determinations from at least four independent experiments. *P < 0.01.
