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Coexpression of GFP-hRFVT-3 (WT) or substrate interaction residue mutants with DsRed-ER in HuTu-80 cells. Coexpression of GFP-hRFVT-3 (WT) and mutants (left) along with DsRed-ER (middle), displayed overlaid images (right). The transiently coexpressing HuTu-80 cells were imaged after 48 h of transfection.
