Fig. 5.

NHERF-1 expression in WKY and SHRs. A: crude membranes from PTCs in primary culture from WKY and SHRs were analyzed by Western blotting using anti-NHERF-1 (top) and NKA α1-subunit (NKA, α6F, bottom). Representative blots from cells from one rat are shown. B: bar graph represents data as ratio of band intensity of NHERF-1 to NKA (means ± SE) from 3 individual rats (n = 3 in each group) as arbitrary units (AU). *P < 0.05 as calculated by one-way ANOVA, followed by Bonferroni post hoc test. C: PTCs in primary culture from WKY and SHRs were treated for 15 min with dopamine (1 μM). Ouabain (4 mM)-sensitive ⁸⁶Rb uptake was used as a measure of NKA activity. Each bar represents data as nmol ⁸⁶Rb·mg protein·⁻¹min⁻¹ (means ± SE) from cell cultures from 3 rats (n = 3 in each group) performed in triplicate. *P < 0.05 as calculated by one-way ANOVA, followed by Bonferroni post hoc test.