Table 1.
Proteomic identification of BBM and BLM proteins from 22-mo-old and 4-mo-old F344 rats
| BBM (22-mo) |
BLM (22-mo) |
BBM (4-mo) |
BLM (4-mo) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Identified Protein | Rat 1 | Rat 2 | Rat 3 | Rat 1 | Rat 2 | Rat3 | Rat 1 | Rat 2 | Rat3 | Rat 1 | Rat 2 | Rat 3 |
| A-kinase anchor protein 2 (Akap2) | 8 | 19 | 0 | 0 | 8 | 0 | 13 | 17 | 31 | 1 | 7 | 3 |
| Aquaporin-1 (Aqp1) | 8 | 7 | 7 | 5 | 9 | 5 | 8 | 8 | 8 | 13 | 8 | 8 |
| Carbonic anhydrase 2 (Ca2) | 14 | 11 | 11 | 1 | 11 | 6 | 14 | 12 | 13 | 11 | 8 | 2 |
| Carbonic anhydrase 4 (Ca4) | 15 | 11 | 11 | 0 | 7 | 2 | 13 | 12 | 10 | 11 | 3 | 8 |
| Caveolin-1 (Cav1) | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 2 | 1 | 1 |
| Ezrin (Ezr) | 40 | 49 | 22 | 14 | 52 | 25 | 40 | 41 | 51 | 39 | 37 | 34 |
| γ-Glutamyltranspeptidase 1 (Ggt1) | 27 | 33 | 31 | 6 | 7 | 2 | 32 | 32 | 28 | 2 | 5 | 8 |
| Glutamyl aminopeptidase (Enpep) | 50 | 52 | 53 | 9 | 5 | 8 | 48 | 46 | 47 | 4 | 2 | 2 |
| Isoform 5 of sodium/calcium exchanger 1 (Slc8a1) isoform 5 | 4 | 5 | 1 | 0 | 7 | 2 | 8 | 4 | 6 | 11 | 2 | 8 |
| Moesin (Msn) | 53 | 70 | 38 | 19 | 65 | 41 | 53 | 64 | 75 | 53 | 57 | 45 |
| Na+/H+ exchange regulatory cofactor NHERF-1 (Slc9a3r1) | 3 | 4 | 9 | 4 | 8 | 7 | 36 | 42 | 51 | 39 | 37 | 23 |
| Na+/H+exchange regulatory cofactor NHERF-2 (Slc9a3r2) | 8 | 6 | 6 | 0 | 0 | 0 | 3 | 4 | 6 | 0 | 0 | 0 |
| Na+/H+exchange regulatory cofactor NHERF-3 (Pdzk1) | 59 | 72 | 26 | 18 | 68 | 18 | 63 | 71 | 73 | 53 | 51 | 44 |
| Sodium-glucose cotransporter 1 (Slc5a1) | 12 | 10 | 11 | 0 | 12 | 2 | 14 | 21 | 13 | 10 | 9 | 8 |
| Sodium-glucose cotransporter 2 (Slc5a2) | 27 | 28 | 23 | 12 | 14 | 15 | 25 | 27 | 27 | 12 | 13 | 10 |
| Sodium/hydrogen exchanger 3 (NHE3) (Slc9a3) | 5 | 2 | 2 | 0 | 1 | 0 | 3 | 4 | 6 | 0 | 1 | 0 |
| Sodium/potassium-transporting ATPase subunit α-1 (Atp1a1) | 10 | 11 | 9 | 78 | 139 | 110 | 13 | 12 | 10 | 197 | 109 | 160 |
| Sodium/potassium-transporting ATPase subunit β-1(Atp1b1) | 36 | 43 | 36 | 21 | 39 | 37 | 41 | 35 | 36 | 46 | 39 | 50 |
| Sodium/potassium-transporting ATPase subunit β-3 (Atp1b3) | 0 | 1 | 2 | 3 | 4 | 5 | 2 | 2 | 1 | 4 | 2 | 4 |
| Sodium/potassium-transporting ATPase subunit γ (Fxyd2) | 2 | 1 | 1 | 4 | 6 | 4 | 1 | 3 | 1 | 5 | 4 | 5 |
| Sodium-dependent phosphate transport protein 2A, NpT2a (Slc34a1) | 8 | 8 | 5 | 1 | 0 | 2 | 8 | 10 | 9 | 1 | 0 | 1 |
| Sodium-dependent phosphate transport protein 2C, NpoT2c (Slc34a3) | 3 | 1 | 1 | 0 | 0 | 0 | 4 | 4 | 5 | 0 | 1 | 1 |
| Solute carrier family 12 member 3 (Slc12a3) | 11 | 15 | 10 | 2 | 1 | 1 | 6 | 8 | 11 | 4 | 1 | 4 |
| Syntaxin-3 (Stx3) | 5 | 6 | 0 | 0 | 3 | 0 | 5 | 6 | 7 | 2 | 0 | 0 |
Relative abundance (based on peptide spectral count values) for selected membrane proteins (and gene name) identified by high confidence proteomic methods (see materials and methods) in brush-border (BBM, apical) and basolateral (BLM) membrane samples isolated from kidneys from three different 4-mo-old and 22-mo-old F344 rats. The numbers in second row are the identification number of the rats. The numbers in columns represent the relative abundance of the proteins based on peptide spectral counts. The complete list of proteins is shown in Supplemental Table S1.