Fig. 6.

CD151 mediates the adhesion of peripheral lymphocytes to HSECs under conditions of shear stress. A: quantification of adherence of lymphocytes to monolayers of HSECs in flow assays pretreated with isotype-matched control (IMC) and CD151, VCAM-1, and a combination of CD151/VCAM-1-blocking antibodies; n = 3 independent experiments with different HSECs. B: proportion of adherent cells that underwent transendothelial migration after blockade with IMC, CD151, VCAM-1, and a combination of CD151/VCAM-1; n = 3 independent experiments with different HSECs. C: representative Western blot analysis of CD151 in HSECs treated with control and CD151 siRNA knockdown. D: quantification of CD151 expression in HSECs treated with siRNA knockdown of CD151 expressed as a % of expression in control HSECs; n = 4 independent experiments with different HSECs. E: quantification of adherence of lymphocytes to monolayers of HSECs in flow assays pretreated with control siRNA and CD151 siRNA, VCAM-1, and a combination of CD151/VCAM-1-blocking antibodies; n = 4 independent experiments with different HSECs. F: representative confocal image of CD151 (red) accumulation around a CD3-positive lymphocyte (blue) firmly adherent to cell tracker green-labeled HSECs. Bar = 5 μm, *P < 0.05, **P < 0.01.