Table 1.
Monoclonal antibodies: clone, source, and final concentration
| Monoclonal Antibodies | Clone | Source | Final Concentration, μg/ml |
|---|---|---|---|
| Mouse negative control | DAK-GO1 | DAKO | 6.7 |
| Mouse negative control | DAK-GO5 | DAKO | 5.0 |
| Mouse negative control | DAK-GO9 | DAKO | 5.0 |
| Mouse anti-human CD151 | IIG5a | Abd Serotec | 6.7 |
| Mouse monoclonal anti-actin α-smooth muscle | 1A4 | Sigma-Aldrich | 5.0 |
| Mouse monoclonal anti-human CD68 | Y1/82A | BD Biosciences | 5.0 |
| Mouse monoclonal anti-human CD90 | SE10 | eBioscience | 1.0 |
| Mouse monoclonal anti-human CD31 | JC70A | DAKO | 5.0 |
| Mouse monoclonal anti-human CD34 | QBEND/10 | Abd Serotec | 5.0 |
| Mouse monoclonal anti-human CD3 | UCHT1 | eBioscience | 4.0 |
| Mouse monoclonal anti-human EpCAM | HEA 125 | Progen Biotechnik | 5.0 |
| Mouse monoclonal anti-human CK18 | DC10 | DAKO | 1.0 |
| Mouse anti-human early endosomes (EEA-1) | 14 | BD Biosciences | 5.0 |
| Mouse anti-human cis-Golgi (GM130) | 35 | BD Biosciences | 5.0 |
| Mouse anti-human lysosome (LAMP-1) | 25 | BD Biosciences | 5.0 |
| Mouse anti-human VCAM-1 | BBA5 | R&D Systems | 5.0 |
| Alexa Fluor 546 goat anti-mouse IgG2a | — | Life Technologies | 8.0 |
| Alexa Fluor 546 goat anti-mouse IgG2b | — | Life Technologies | 8.0 |
| Alexa Fluor 546 goat anti-rat IgG2a | — | Life Technologies | 8.0 |
EpCAM, epithelial cell adhesion molecule.