Table 3.
Total levels of other lipid species in intestine and feces assessed by HPLC-MS
| Upper Small Intestine |
Lower Small Intestine |
Large Intestine |
Feces |
|||||
|---|---|---|---|---|---|---|---|---|
| Control | Cyp8b1−/− | Control | Cyp8b1−/− | Control | Cyp8b1−/− | Control | Cyp8b1−/− | |
| MG | 3.30 ± 0.14 | 4.12 ± 0.19† | 7.37 ± 0.35 | 6.32 ± 0.37 | 9.09 ± 0.94 | 6.65 ± 0.44* | 2.76 ± 0.96 | 9.63 ± 7.03 |
| DG | 11.86 ± 1.89 | 14.75 ± 1.15 | 18.51 ± 2.49 | 8.67 ± 1.74† | 8.86 ± 1.68 | 5.15 ± 0.44* | 1.67 ± 0.44 | 4.95 ± 2.08 |
| TG | 36.63 ± 6.01 | 59.34 ± 10.22 | 135.84 ± 20.79 | 70.68 ± 10.77† | 69.39 ± 10.73 | 31.72 ± 4.95† | 0.46 ± 0.13 | 2.10 ± 0.91 |
| SM | 12.70 ± 0.69 | 14.05 ± 0.92 | 8.36 ± 0.87 | 5.10 ± 0.70† | 4.48 ± 0.43 | 3.32 ± 0.32* | 0.06 ± 0.02 | 0.75 ± 0.51 |
| PC | 32.35 ± 2.26 | 34.46 ± 1.38 | 23.38 ± 1.89 | 15.38 ± 1.72† | 14.68 ± 1.56 | 11.82 ± 1.27 | 0.09 ± 0.05 | 0.44 ± 0.20 |
| PE | 11.80 ± 1.08 | 10.99 ± 0.58 | 0.36 ± 0.03 | 0.20 ± 0.04† | 0.19 ± 0.03 | 0.16 ± 0.02 | 0.01 ± 0.01 | 0.01 ± 0.01 |
Values are means ± SE and given as nanomoles per milligram. MG, monoacylglycerol; DG, diacylglycerol; TG, triacylglycerol; SM, sphingomyelin; PC, phosphatidylcholine; PE, phosphatidylethanolamine. MG group is composed of MG 16:0, MG 16:1, MG 18:0, MG 18:1, MG 18:2, MG 18:3, MG 20:0, MG 20:2, MG 20:3, MG 20:4, MG 22:0, MG 22:3, MG 22:4, and MG 22:5. DG group is composed of DG 28:0/14:0, DG 30:0/14:0, DG 30:1/14:0, DG 32:0/16:0, DG 32:1/16:0, DG 32:2/16:1, DG 34:0/16:0, DG 34:1/16:0, DG 34:2/16:0, DG 34:2/16:1, DG 36:0/18:0, DG 36:1/18:0, DG 36:2/18:0, DG 36:2/18:1, DG 36:3/18:1, DG 36:4/18:0, DG 38:2/18:0, DG 38:2/18:1, DG 38:3/18:0, DG 38:3/18:1, DG 38:4/18:0, DG 38:4/18:1, DG 40:4/18:0, DG 40:5/18:0, DG 40:5/18:1, DG 40:6/18:0, and DG 40:6/18:1. TG group is composed of TG 48:0/16:0, TG 48:1/16:0, TG 50:0/16:0, TG 50:1/16:1, TG 50:2/16:1, TG 52:0/18:0, TG 52:1/18:0, TG 52:2/18:0, TG 52:3/18:1, TG 52:4/18:1, TG 52:5/20:4, TG 54:0/18:0, TG 54:1/18:0, TG 54:2/18:0, TG 54:3/18:0, TG 54:4/18:1, TG 54:4/20:4, TG 54:5/18:1, TG 54:6/18:1, TG 54:6/20:4, TG 54:7/18:1, TG 56:4/18:1, TG 56:4/20:4, TG 56:5/18:1, TG 56:8/20:4, TG 56:9/20:4, TG 58:7/20:4, TG 58:8/22:6, and TG 58:9/22:6. SM group is composed of SM d18:1/16:0, SM d18:1/16:1, SM d18:1/18:0, SM d18:1/18:1, SM d18:1/20:0, SM d18:1/20:1, SM d18:1/22:0, SM d18:1/22:1, SM d18:1/24:0, and SM d18:1/24:1. PC group is composed of PC 30:0, PC 32:0, PC 32:1, PC 34:0, PC 34:1, PC 34:2, PC 36:0, PC 36:1, PC 36:2, PC 36:3, PC 36:4, PC 38:1, PC 38:2, PC 38:3, PC 38:4, PC 38:5, PC 38:6, PC 40:4, PC 40:5, PC 40:6, and PC 40:7. PE group is composed of PE 30:0, PE 32:0, PE 32:1, PE 34:0, PE 34:1, PE 34:2, PE 36:0, PE 36:1, PE 36:2, PE 36:3, PE 36:4, PE 38:0, PE 38:1, PE 38:2, PE 38:3, PE 38:4, PE 38:5, PE 38:6, PE 40:4, PE 40:5, and PE 40:6.
P < 0.05,
P < 0.01 in Cyp8b1−/− vs. controls measured by Student’s t-test (n = 8).