Fig. 2.

Menin reduces GLP1R agonist and forskolin-induced phosphorylation of FOXO1. A: two different clones of either menin-overexpressing or vector-transfected MIN6 cells were generated. MIN6 cells were starved in serum-free, low-glucose medium for 48 h and then treated with the drugs indicated for 30 min. Representative Western blots (B) and quantitation (means ± SE) (C) of Western blots from three similar experiments demonstrating that menin overexpression blunted phosphorylation of FOXO1 (pFOXO1-S253) in response to treatment with either increasing concentrations of exendin-4 (0, 0.02, 2, and 200 nM) or forskolin (10 μM) (lanes 7 to 12) compared with vector-transfected controls (lanes 1 to 6). Data sets were analyzed using two-way repeated-measures ANOVA, *P < 0.05.