Fig. 6.
PRMT5 interacts with FOXO1 and CREB. Coimmunoprecipitation of PRMT5 from MIN6 cells using antibodies against CREB and FOXO1 for the immunoprecipitation followed by Western blot analyses with antibodies against PRMT5, FOXO1, or CREB. Representative blots (A) and quantitation of PRMT5 pulldown compared with IgG controls (B; results expressed as means ± SE from three similar experiments; *P < 0.05), showing PRMT5 is enriched in both anti-FOXO1 (lane 2) and anti-CREB (lane 3) antibody groups compared with IgG control (lanes 4). C: proximity ligation assay in vector-transfected menin-null (Men1Δ/Δ) MEF cells and menin-complemented (Men1Δ/Δ+Menin) MEF cells using anti-PRMT5 and anti-CREB antibodies, shows colocalization of PRMT5 with CREB, and menin complementation increases the frequency of this association (two similar experiments).