Skip to main content
. 2017 Mar 7;313(2):E148–E166. doi: 10.1152/ajpendo.00241.2016

Table 1.

Primer sequences used for qPCR

Target Gene Primer Sequence
Mouse primers
    GLP1R primer pair 1 Forward 5′ ACG GTG TCC CTC TCA GAG AC 3′
Reverse 5′ ATC AAA GGT CCG GTT GCA GAA 3′
    GLP1R primer pair 2 Forward 5′ CCC AGG TTC CTT CGT GAA TGT 3′
Reverse 5′ GTT GTC CTT ATG TAG CCA GAG AC 3′
    Menin primer pair 1 Forward 5′ GGA CCC AGA ATG CTT TGC C 3′
Reverse 5′ GAC TGC ACA AGG AAA GTT GCC 3′
    Menin primer pair 2 Forward 5′ GAA AGA TAC CCC ATG GCG CTA 3′
Reverse 5′ TTT CGG TTG CGA CAG TGG TAG 3′
    IRS2 primer pair 1 Forward 5′ GTG GGT TTC CAG AAC GGC CT 3′
Reverse 5′ ATG GGG CTG GTA GCG CTT CA 3′
    IRS2 primer pair 2 Forward 5′ AGT AAA CGG AGG TGG CTA CA 3′
Reverse 5′ AAG CTG CTG AGA AGT CAG GT 3′
    NR4A2 Forward 5′ GCA TAC AGG TCC AAC CCA GT 3′
Reverse 5′ AAT GCA GGA GAA GGC AGA AA 3′
    Cyclin A2 Forward 5′ GCG CTG TGC ACG CTC TG 3′
Reverse 5′ TCC TTA AGA GGA GCA ACC CGT 3′
    HPRT1 Forward 5′ TGC TCG AGA TGT CAT GAA GG 3′
Reverse 5′ TAT GTC CCC CGT TGA CTG AT 3′
    FOXO1 Forward 5′ CAG CCG CGC AAG ACC AGC TC 3′
Reverse 5′ TTG AAT TCT TCC AGC CCG CCG AG 3′
Human primers
    GLP1R (25) Forward 5′ TTG GGG TGA ACT TCC TCA TC 3′
Reverse 5′ CTT GGC AAG TCT GCA TTT GA 3′
    Ki67 primer pair 1 Forward 5′ TAC GTG AAC AGG AGC CAG CA 3′
Reverse 5′ GTT CCC TGA GCA ACA CTG TC 3′
    Ki67 primer pair 2 Forward 5′ TCT GAC CCT GAT GAG AAA GCT C 3′
Reverse 5′ AGT TGT TCC CTG AGC AAC AC 3′
Primers used to amplify chromatin immunoprecipitation DNA product
    Amplicon 1 Forward 5′ GGT GGC ACC CAA CTA GTC CGA 3′
Reverse 5′ GCC CCC ACC GAG TCA GTG CT 3′
    Amplicon 2 Forward 5′ AGG AGG AGA TCG GGG TGC GG 3′
Reverse 5′ ATG TTC GGC GCT GTG CTC CC 3′