Fig. 2.

ARG2 expression and activity increased after renal IRI. Both left and right renal pedicles were clamped for 28 min in 7- to 9-wk-old anesthetized mice. Plasma and kidney tissue were collected at the indicated time point following ischemia. Kidney function was assessed for BUN (A) and plasma creatinine levels (B). Quantitative RT-PCR was performed on kidney RNA for Arg2 (C), and Arg1 (D) expression. ARG1 (E) and ARG2 (E and F) protein levels were determined using Western blot. E: representative Western blot of samples for the time course in F. Kidney (G) and plasma (H) arginase activity were determined as described in materials and methods. Values are means ± SE for n = 5 mice/time point. *P < 0.05, **P < 0.01, and ***P < 0.005 vs. 0 h.