Fig. 9.

Arg2 deficiency or arginase inhibition increased kidney NO after IRI. Levels of nitrate and nitrite and Western blot for total and p-eNOS were determined in whole kidney tissue 24 h after IRI in WT and Arg2^−/− mice (A) or BEC-treated mice (B). p-eNOS-to-eNOS ratios in each experiment were normalized to the average value for sham WT or vehicle controls and arbitrarily set to 1.0. Values are means ± SE for n = 6 mice in each group. *P < 0.05 and **P < 0.01 vs. sham. #P < 0.05 and ##P < 0.01 vs. vehicle-treated or WT IRI.