Table 1.
Primer Sequence | |
---|---|
β-Actin | |
Forward | 5′-GTCCCTCACCCTCCCAAAAG-3′ |
Reverse | 5′-GCTGCCTCAACACCTCAACCC-3′ |
AT1 | |
Forward | 5′-ATCGCTACCTGGCCATTGTC-3′ |
Reverse | 5′-GGAAGCCCAGGATGTTCTTG-3′ |
AT2 | |
Forward | F 5′-TTACCAGCAGCCGTCCTTTT-3′ |
Reverse | 5′-GTCAGCCAAGGCCAGATTGA-3′ |
ET-1 | |
Forward | 5′-TCTTCCAGGTCCAAGCGTTC-3′ |
Reverse | 5′-TGCTATTGCTGATGGCCTCC-3′ |
ETA | |
Forward | 5′-TTGACCTCCCCATCAACGTG-3′ |
Reverse | 5′-AGCACAGAGGTTCAAGACGG-3′ |
ETB | |
Forward | 5′-CAAGGTCGCTCAGAAAACGC-3′ |
Reverse | 5′-CCTAAACAGGCCTCTCGCAA-3′ |
NHE3 | |
Forward | 5′-GCCTTCATTCGCTCCCCAAGT-3′, |
Reverse | 5′-GAGATGCTTGTACTCCTGCCGA-3′. |
NCC | |
Forward | 5′-CCATTGGAAGGAAGGGGAAGTGC-3′ |
Reverse | 5′-GTGCGTTCTGACTCTATCCCA-3′. |
NKCC2 | |
Forward | 5′-GATGCAGAACTGGAAGCAGTCAA-3′ |
Reverse | 5′-TCAGACACCAAGGCACAACATTT-3′ |
α-ENaC | |
Forward | 5′-CGGGAAACGACCAAACGAAC-3′ |
Reverse | 5′-CTTACTCTAGCCCCCACCCT-3′ |
β-ENaC | |
Forward | 5′-TTGATGAGCGGAACCCTGAC3′ |
Reverse | 5′-GGATTATGCGATCAGGGGCA3′ |
γ-ENaC | |
Forward | 5′-CCTGGAGAGAAGATCAAAGCCA-3′ |
Reverse | 5′-CCAAGTCAGTCAGGAGGTCAC-3′ |
IL-6 | |
Forward | 5′-CTCTGGGAAATCGTGGAAAT-3′, |
Reverse | 5′-CCAGTTTGGTAGCATCCATC-3′. |
KC | |
Forward | 5′-GCTGGGATTCACCTCAAGAA-3′, |
Reverse | 5′-TGGGGACACCTTTTAGCATC-3′. |
TGF-β | |
Forward | 5′-CCCTATATTTGGAGCCTGGA-3′, |
Reverse | 5′-CTTGCGACCCACGTAGTAGA-3′ |
TNF-α | |
Forward | 5′-ATGAGAAGTTCCCAAATGGC-3′, |
Reverse | 5′-CTCCACTTGGTGGTTTGCTA-3′ |
INF-γ | |
Forward | 5′-CGCTACACACTGCATCTTGG-3′, |
Reverse | 5′-GGCTGGATTCCGGCAACA-3′. |
IL-17 | |
Forward | 5′-ATCAGGACGCGCAAACATG-3′, |
Reverse | 5′-TGATCGCTGCTGCCTTCAC-3′. |
CCL2 | |
Forward | 5′-AGTTAACGCCCCACTCACCT-3′, |
Reverse | 5′-CCATTCCTTCTTGGGGTCAGC-3′ |
CCL5 | |
Forward | 5′-AGATCTCTGCAGCTGCCCTCA-3′, |
Reverse | 5′-GGAGCACTTGCTGCTGGTGTAG-3′ |
AT1 and AT2, angiotensin II receptors; ET, endothellin; NHE3, Na+/H+ exchanger 3; NCC, Na+-Cl− cotransporter; NKCC2, Na+-K−-2Cl cotransporter 2; ENaC, epithelial Na+ channels; TGF-β, transforming growth factor-β; KC, keratinocyte-derived chemokine; CCL, chemokine (C-C motif) ligand.