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. 2017 May 17;313(2):F326–F338. doi: 10.1152/ajprenal.00039.2017

Table 1.

Primers

Primer Sequence
β-Actin
    Forward 5′-GTCCCTCACCCTCCCAAAAG-3′
    Reverse 5′-GCTGCCTCAACACCTCAACCC-3′
AT1
    Forward 5′-ATCGCTACCTGGCCATTGTC-3′
    Reverse 5′-GGAAGCCCAGGATGTTCTTG-3′
AT2
    Forward F 5′-TTACCAGCAGCCGTCCTTTT-3′
    Reverse 5′-GTCAGCCAAGGCCAGATTGA-3′
ET-1
    Forward 5′-TCTTCCAGGTCCAAGCGTTC-3′
    Reverse 5′-TGCTATTGCTGATGGCCTCC-3′
ETA
    Forward 5′-TTGACCTCCCCATCAACGTG-3′
    Reverse 5′-AGCACAGAGGTTCAAGACGG-3′
ETB
    Forward 5′-CAAGGTCGCTCAGAAAACGC-3′
    Reverse 5′-CCTAAACAGGCCTCTCGCAA-3′
NHE3
    Forward 5′-GCCTTCATTCGCTCCCCAAGT-3′,
    Reverse 5′-GAGATGCTTGTACTCCTGCCGA-3′.
NCC
    Forward 5′-CCATTGGAAGGAAGGGGAAGTGC-3′
    Reverse 5′-GTGCGTTCTGACTCTATCCCA-3′.
NKCC2
    Forward 5′-GATGCAGAACTGGAAGCAGTCAA-3′
    Reverse 5′-TCAGACACCAAGGCACAACATTT-3′
α-ENaC
    Forward 5′-CGGGAAACGACCAAACGAAC-3′
    Reverse 5′-CTTACTCTAGCCCCCACCCT-3′
β-ENaC
    Forward 5′-TTGATGAGCGGAACCCTGAC3′
    Reverse 5′-GGATTATGCGATCAGGGGCA3′
γ-ENaC
    Forward 5′-CCTGGAGAGAAGATCAAAGCCA-3′
    Reverse 5′-CCAAGTCAGTCAGGAGGTCAC-3′
IL-6
    Forward 5′-CTCTGGGAAATCGTGGAAAT-3′,
    Reverse 5′-CCAGTTTGGTAGCATCCATC-3′.
KC
    Forward 5′-GCTGGGATTCACCTCAAGAA-3′,
    Reverse 5′-TGGGGACACCTTTTAGCATC-3′.
TGF-β
    Forward 5′-CCCTATATTTGGAGCCTGGA-3′,
    Reverse 5′-CTTGCGACCCACGTAGTAGA-3′
TNF-α
    Forward 5′-ATGAGAAGTTCCCAAATGGC-3′,
    Reverse 5′-CTCCACTTGGTGGTTTGCTA-3′
INF-γ
    Forward 5′-CGCTACACACTGCATCTTGG-3′,
    Reverse 5′-GGCTGGATTCCGGCAACA-3′.
IL-17
    Forward 5′-ATCAGGACGCGCAAACATG-3′,
    Reverse 5′-TGATCGCTGCTGCCTTCAC-3′.
CCL2
    Forward 5′-AGTTAACGCCCCACTCACCT-3′,
    Reverse 5′-CCATTCCTTCTTGGGGTCAGC-3′
CCL5
    Forward 5′-AGATCTCTGCAGCTGCCCTCA-3′,
    Reverse 5′-GGAGCACTTGCTGCTGGTGTAG-3′

AT1 and AT2, angiotensin II receptors; ET, endothellin; NHE3, Na+/H+ exchanger 3; NCC, Na+-Cl cotransporter; NKCC2, Na+-K-2Cl cotransporter 2; ENaC, epithelial Na+ channels; TGF-β, transforming growth factor-β; KC, keratinocyte-derived chemokine; CCL, chemokine (C-C motif) ligand.