Fig. 3.

Cyclosporine A (CsA) does not prevent VP-induced dephosphorylation of S261. A: specific antibodies against pS261 were used on Western blots with cell lysates of LLC-AQP2 cells treated with CsA (70 nM), VP (10 nM), or CsA + VP. B: intensities of phosphoserine bands were normalized to the band intensity of total AQP2. CsA did not prevent dephosphorylation of S261 after VP treatment. Values are means ± SE (n = 5). **P < 0.01, ***P < 0.001. C: PKA activity was not altered by CsA. Values are means ± SE (n = 3). **P < 0.01, ***P < 0.001, ****P < 0.0001 (by 1-way ANOVA with Tukey’s test).